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Information about Respirometer

A respirometer is a device used to measure the rate of respiration of a living organism by measuring its rate of exchange of oxygen and carbon dioxide. They allow investigation into how factors such as age or the effect of light affect the rate of respiration.

A simple respirometer designed to measure oxygen uptake or CO2 release consists of a sealed container with the living specimen together with a substance to absorb the carbon dioxide given off during respiration, such as soda lime pellets or cotton wads soaked with potassium hydroxide. The oxygen uptake is detected by displacement of manometric fluid in a thin glass U-tube connected to the container. When the organism takes in oxygen it gives off an equal volume of carbon dioxide. As this is absorbed by the soda lime, air is sucked in from the U-tube to keep the pressure constant, displacing the liquid. The rate of change gives a direct and reasonably accurate reading for the organism's rate of respiration.

As changes in temperature or pressure can also affect the displacement of the manometric fluid, a second respirometer identical to the first except with a dead specimen (or something with the same mass as the specimen in place of the organism) is sometimes set up. Subtracting the displacement of the second respirometer from the first allows for control of these factors.

See also

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Respiratory system, physiology: respiratory physiology
Volumeslung volumes - vital capacity - functional residual capacity - respiratory minute volume - dead space - spirometry - body plethysmography - peak flow meter
Airwaysventilation (V) (positive pressure) - breath (inhalation, exhalation) -respiratory rate - respirometer - pulmonary surfactant - compliance - hysteresivity - airway resistance
Bloodpulmonary circulation - perfusion (Q) - hypoxic pulmonary vasoconstriction - pulmonary shunt
Interactionsventilation/perfusion ratio (V/Q) and scan - zones of the lung - gas exchange - pulmonary gas pressures - alveolar gas equation - hemoglobin - oxygen-haemoglobin dissociation curve (2,3-DPG, Bohr effect, Haldane effect) - carbonic anhydrase (chloride shift) - oxyhemoglobin - respiratory quotient - arterial blood gas - diffusion capacity - Dlco
Control of respirationpons (pneumotaxic center, apneustic center) - medulla (dorsal respiratory group, ventral respiratory group) - chemoreceptors (central, peripheral) - pulmonary stretch receptors - Hering-Breuer reflex
Insufficiencyhigh altitude - oxygen toxicity - hypoxia
In animal physiology, respiration is the transport of oxygen from the ambient air to the tissue cells and the transport of carbon dioxide in the opposite direction. This is in contrast to the biochemical definition of respiration, which refers to cellular respiration
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2, −1
(neutral oxide)
Electronegativity 3.44 (Pauling scale)
Ionization energies
(more) 1st: 1313.9 kJmol−1
2nd: 3388.3 kJmol−1
3rd: 5300.5 kJmol−1

Atomic radius 60 pm
Atomic radius (calc.
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Carbon dioxide is a chemical compound composed of two oxygen atoms covalently bonded to a single carbon atom. It is a gas at standard temperature and pressure and exists in Earth's atmosphere in this state.
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Soda lime is a mixture of chemicals, used in granular form in closed breathing environments, such as general anaesthesia, submarines, rebreathers and recompression chambers, to remove carbon dioxide from breathing gases to prevent CO2
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Cotton is a soft fibre that grows around the seeds of the cotton plant (Gossypium sp.), a shrub native to tropical and subtropical regions around the world, including the Americas, India, and Africa.
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The chemical compound potassium hydroxide (KOH), sometimes known as caustic potash, potassa, potash lye, and potassium hydrate, is a metallic base.
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pressure gauges or vacuum gauges

A manometer is a pressure measuring instrument, usually limited to measuring pressures near to atmospheric. The term manometer is often used to refer specifically to liquid column hydrostatic instruments.
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respiratory rate (or respiration rate) is the number of breaths a living being, such as a human, takes per minute.

There is only limited research on monitoring alien respiratory rate, and these studies have focused on such issues as the inaccuracy of respiratory rate
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respiratory system generally includes tubes, such as the bronchi, used to carry air to the lungs, where gas exchange takes place. A diaphragm pulls air in and pushes it out. Respiratory systems of various types are found in a wide variety of organisms.
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Human physiology is the science of the mechanical, physical, and biochemical functions of humans in good health, their organs, and the cells of which they are composed. The principal level of focus of physiology is at the level of organs and systems.
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Respiratory physiology is the branch of human physiology focusing upon respiration.

Topics include:

Volumes

  • lung volumes
  • vital capacity
  • functional residual capacity
  • dead space
  • spirometry
  • body plethysmography
  • peak flow meter


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Larger volumes Smaller volumes
males females
taller people shorter people
non-smokers heavy smokers
professional athletes[1] non-athletes
people living at high altitudes people living at low altitudes
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Vital capacity is the maximum volume of air that a person can exhale after maximum inhalation. It can also be the maximum volume of air that a person can inhale after maximum exhalation.
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Functional Residual Capacity (FRC) is the volume of air present in the lungs at the end of passive expiration. At FRC, the elastic recoil forces of the lungs and chest wall are equal but opposite and there is no exertion by the diaphragm or other respiratory muscles.
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Respiratory minute volume (or minute ventilation, or flow of gas) is the volume of air which can be inhaled (inhaled minute volume) or exhaled (exhaled minute volume) from a person's lungs in one minute.
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dead space is air that is inhaled by the body in breathing, but does not partake in gas exchange.

In adults, it is usually in the range of 150ml.[1]

Understanding dead space


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Spirometry (meaning the measuring of breath) is the most common of the Pulmonary Function Tests (PFTs), measuring lung function, specifically the measurement of the amount (volume) and/or speed (flow) of air that can be inhaled and exhaled.
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A peak flow meter is a small, hand-held device used to manage asthma by monitoring airflow through the bronchi and thus the degree of restriction in the airways. The peak flow meter measures the patient's maximum ability to expel air from the lungs, or
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ventilation (or ventilation rate) is the rate at which gas enters or leaves the lung. It is categorised under the following definitions:

Measurement Equation Description
Minute ventilation = tidal volume * respiratory rate[1]
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Positive pressure ventilators help patients with respiratory problems to breathe easier. They use high pressure gas at the opening of the patients lungs in order to mobilize oxygen flow down the pressure gradient, and into the patient's lungs.
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Breathing transports oxygen into the body and carbon dioxide out of the body. Aerobic organisms require oxygen to create energy via respiration, in the form of energy-rich molecules such as glucose. The medical term for normal relaxed breathing is eupnoea.
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Inhalation (also known as inspiration) is the movement of air from the external environment, through the airways, into the alveoli during breathing.

Inhalation begins with the onset of contraction of the diaphragm, which results in expansion of the intrapleural space
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Exhalation (or expiration) is the movement of air out of the bronchial tubes, through the airways, to the external environment during breathing.

Exhaled air is rich in carbon dioxide, a waste product of cellular respiration during the production of ATP.
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respiratory rate (or respiration rate) is the number of breaths a living being, such as a human, takes per minute.

There is only limited research on monitoring alien respiratory rate, and these studies have focused on such issues as the inaccuracy of respiratory rate
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Pulmonary surfactant is a surface-active lipoprotein complex formed by type II alveolar cells. The proteins and lipids that comprise surfactant have both a hydrophilic region and a hydrophobic region.
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158 (4): 1052-60. PMID 9769260.
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“Hysteresivity” derives from “hysteresis”, meaning “lag”. It is the tendency to react slowly to an outside force, or to not return completely to its original state.
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Airway resistance is a concept used in respiratory physiology to describe mechanical factors which limit the access of inspired air to the pulmonary alveoli, and thus determine airflow.
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Pulmonary circulation is the portion of the cardiovascular system which carries oxygen-depleted blood away from the heart, to the lungs, and returns oxygenated blood back to the heart. The term is contrasted with systemic circulation.
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