Information about Electroantenogram

Electroantennogram or EAG is a technique by which we measure the average output of the antenna to the brain for a given odor. It is commonly used in the electrophysiology while studying the function of olfactory pathway in insects. The technique was invented in 1957 by German biologist Dietrich Schneider.[1]

EAG is performed in two ways:
  1. Remove the antenna from the animal and insert two wires for contact on to the two ends and amplify the voltage between them.
  2. Leave the animal intact and insert a ground wire to some part of the body and another wire to the tip of the antenna. The later method is useful if one is doing an experiment on the animal as a whole while doing the antennogram.


The technique is widely applied in screening of insect pheromones by examining the responses to fractions of a compound mixture separated using chromatography.[2]

Usually the wire inserted into the antenna is a thin silver wire which is chlorided in bleach.

References

1. ^ National Academy of sciences (2003). Beyond discovery - Insect pheromones.
2. ^ Hummel, H. E. and T. A. Miller (1984). Techniques in pheromone research.Springer-Verlag,New York.
Antennae (singular antenna) are paired appendages connected to the front-most segments of arthropods. In crustaceans, they are biramous and present on the first two segments of the head, with the smaller pair known as antennules.
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In animals, the brain or encephalon (Greek for "in the skull"), is the control center of the central nervous system, responsible for behavior. The brain is located in the head, protected by the skull and close to the primary sensory apparatus of vision, hearing,
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odor or odour (see spelling differences) is a volatilized chemical compound, generally at a very low concentration, which humans and other animals perceive by the sense of olfaction. Odors are also called smells, which can refer to both pleasant and unpleasant odors.
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Electrophysiology is the study of the electrical properties of biological cells and tissues. It involves measurements of voltage change or electrical current flow on a wide variety of scales from single ion channel proteins to whole tissues like the heart.
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Olfaction (also known as olfactics) refers to the sense of smell. This sense is mediated by specialized sensory cells of the nasal cavity of vertbrates, and, by analogy, sensory cells of the antennae of invertebrates.
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Insecta
Linnaeus, 1758

Orders
Subclass Apterygota
* Archaeognatha (bristletails)
* Thysanura (silverfish)
Subclass Pterygota
* Infraclass Paleoptera (Probably paraphyletic)

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Antennae (singular antenna) are paired appendages connected to the front-most segments of arthropods. In crustaceans, they are biramous and present on the first two segments of the head, with the smaller pair known as antennules.
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pheromone is a chemical that triggers a natural behavioral response in another member of the same species. There are alarm pheromones, food trail pheromones, sex pheromones, and many others that affect behavior or physiology.
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Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures.
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