Information about Sudan Black B
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Sudan Black B
Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.
Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.
A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
Its CAS number is [] and its SMILES structure is CC(N6)(C)NC1=CC=C (N=NC3=C(C=CC=C5) C5=C(N=NC4=CC=CC=C4) C=C3)C2=C1C6=CC=C2.
Its other names are Sudan Black, Fat Black HB, Solvent Black 3, and C.I. 26150.
External links
- Stains File entry
A lysochrome is a soluble dye used for biochemical staining of triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV bind to the lipid in a substrate and show up as colored regions.
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Azo compounds refer to synthetic inorganic chemical compounds bearing the functional group R-N=N-R', in which R and R' can be either aryl or alkyl. The N=N group is called an azo or diimide.
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Staining is a biochemical technique of adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. It is similar to fluorescent tagging.
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Triglyceride (more properly known as triacylglycerol or triacylglyceride
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Lipids can be broadly defined as any fat-soluble (hydrophobic), naturally-occurring molecules. The term is more-specifically used to refer to fatty-acids and their derivatives (including tri-, di-, and monoglycerides and phospholipids) as well as other fat-soluble sterol-containing
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A lipoprotein is a biochemical assembly that contains both proteins and lipids. The lipids or their derivatives may be covalently or non-covalently bound to the proteins. Many enzymes, transporters, structural proteins, antigens, adhesins and toxins are lipoproteins.
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Sudan staining is the use of Sudan dyes to stain sudanophilic substances, usually lipids. Sudan lysochromes (Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B) are used.
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Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C26H24N4O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections.
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Sudan III is a lysochrome (fat-soluble dye) diazo dye used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507(304) nm.
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Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for staining of lipids, triglycerides and lipoproteins on frozen paraffin sections.
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CAS registry numbers are unique numerical identifiers for chemical compounds, polymers, biological sequences, mixtures and alloys. They are also referred to as CAS numbers, CAS RNs or CAS #s.
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smiles
File extension:
Type of format: chemical file format
The simplified molecular input line entry specification or SMILES
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File extension:
.smiType of format: chemical file format
The simplified molecular input line entry specification or SMILES
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