Information about Nitrilotriacetic Acid

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Skeletal formula of nitrilotriacetic acid
Nitrilotriacetic acid (NTA), C6H9NO6, is a chemical compound used as a chelating agent which forms coordination compounds with metal ions (chelates) such as Ca2+, Cu2+ or Fe3+. In 1999 about 20,000 tons of NTA were used in Europe.

The uses of NTA are similar to that of EDTA.

In biotechnology applications, NTA is combined with His-tags.

In contrast to EDTA, NTA is easily biodegradable and is almost completely removed during wastewater treatment.

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Chelation (from Greek χηλή, chelè, meaning claw; pronounced [ˌki:ˈleɪʃən]) is the binding or complexation of a bi- or multidentate ligand.
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complex in chemistry usually is used to describe molecules or ensembles formed by the combination of ligands and metal ions. Originally, a complex implied a reversible association of molecules, atoms, or ions through weak chemical bonds.
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EDTA is a widely-used acronym for the chemical compound ethylenediamine tetraacetic acid (and many other names, see table). EDTA refers to the chelating agent with the formula (HO2CCH2)2NCH2CH2N(CH2CO
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polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag
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