Information about Myasthenia Gravis

Myasthenia gravis
Classification & external resources

Global view of a neuromuscular junction: 1. Axon 2. Motor end-plate 3. Muscle fiber 4. Myofibril
ICD-10	G70.0
ICD-9	358.0
OMIM	254200
DiseasesDB	8460
MedlinePlus	000712
eMedicine	neuro/232  emerg/325 -emergency, med/3260 -pregnancy, oph/263 -eye
MeSH	D009157

Myasthenia gravis (sometimes abbreviated MG; from the Greek myastheneia, lit. 'condition of no strength in the muscle', and Latin gravis, 'serious') is a neuromuscular disease leading to fluctuating muscle weakness and fatiguability. At 20 cases per 100,000 (in the U.S.),^[1] it is one of the lesser known autoimmune disorders. Weakness is typically caused by circulating antibodies that block acetylcholine receptors at the post-synaptic neuromuscular junction,^[2] inhibiting the stimulative effect of the neurotransmitter acetylcholine. Myasthenia is treated with immunosuppressants, cholinesterase inhibitors and, in selected cases, thymectomy.

Classification

Myasthenia Gravis Foundation of America Clinical Classification

• Class I: Any eye muscle weakness Possible ptosis No other evidence of muscle weakness elsewhere
• Class II: Eye muscle weakness of any severity Mild weakness of other muscles
• Class IIa: Predominantly limb or axial muscles
• Class IIb: Predominantly bulbar and/or respiratory muscles
• Class III: Eye muscle weakness of any severity Moderate weakness of other muscles
• Class IIIa: Predominantly limb or axial muscles
• Class IIIb: Predominantly bulbar and/or respiratory muscles
• Class IV: Eye muscle weakness of any severity Severe weakness of other muscles
• Class IVa: Predominantly limb or axial muscles
• Class IVb: Predominantly bulbar and/or respiratory muscles (Can also include feeding tube without intubation)
• Class V: Intubation to maintain airway

Signs and symptoms

The hallmark of myasthenia gravis is muscle weakness that increases during periods of activity and improves after periods of rest. Muscles that control eye and eyelid movement, facial expression, chewing, talking, and swallowing are especially susceptible. The muscles that control breathing and neck and limb movements can also be affected. Often the physical examination is within normal limits.^[3]

The onset of the disorder can be sudden or rapid. Often symptoms come and go over time. The diagnosis of myasthenia gravis is often initially missed.

In most cases, the first noticeable symptom is weakness of the eye muscles. In others, difficulty in swallowing and slurred speech may be the first signs. The degree of muscle weakness involved in MG varies greatly among patients, ranging from a localized form, limited to eye muscles (ocular myasthenia), to a severe or generalized form in which many muscles - sometimes including those that control breathing - are affected. Symptoms, which vary in type and severity, may include asymmetrical ptosis (a drooping of one or both eyelids), diplopia (double vision) due to weakness of the muscles that control eye movements, unstable or waddling gait, weakness in arms, hands, fingers, legs, and neck, a change in facial expression, dysphagia (difficulty in swallowing), shortness of breath and dysarthria (impaired speech, often nasal due to weakness of the pharyngeal muscles).

In myasthenic crisis a paralysis of the respiratory muscles occurs, necessitating assisted ventilation to sustain life. In patients whose respiratory muscles are already weak, crises may be triggered by infection, fever, an adverse reaction to medication, or emotional stress.^[4] Since the heart muscle is stimulated differently, it is never affected by MG.

Pathophysiology

Myasthenia gravis is an autoimmune disease: it features antibodies directed against the body's own proteins. While in various similar diseases the disease has been linked to a cross-reaction with an infective agent, there is no known causative pathogen that could account for myasthenia. There is a slight genetic predisposition: particular HLA types seem to predispose for MG (B8 and DR3 with DR1 more specific for ocular myasthenia). Up to 25% have a concurrent thymoma, a tumor (either benign or malignant) of the thymus, and other abnormalities are frequently found. The disease process generally remains stationary after thymectomy (removal of the thymus).

In MG, the autoantibodies are directed most commonly against the acetylcholine receptor (nicotinic type), the receptor in the motor end plate for the neurotransmitter acetylcholine that stimulates muscular contraction. Some forms of the antibody impair the ability of acetylcholine to bind to receptors. Others lead to the destruction of receptors, either by complement fixation or by inducing the muscle cell to eliminate the receptors through endocytosis.

The antibodies are produced by plasma cells, that have been derived from B cells. These plasma cells are activated by T-helper cells, which in turn are activated by binding to acetylcholine receptor antigenic peptide sequences (epitopes) that rest within the histocompatibility antigens of antigen presenting cells. The thymus plays an important role in the development of T-cells, which is why myasthenia gravis is associated with thymoma. The exact mechanisms are however not convincingly clarified.

In normal muscle contraction, cumulative activation of the ACh receptor leads to influx of sodium and calcium. Only when the levels of these electrolytes inside the muscle cell is high enough will it contract. Decreased numbers of functioning receptors therefore impairs muscular contraction.

It has recently been realized that a second category of gravis is due to auto-antibodies against the MuSK receptor (Muscle Specific Kinase), a tyrosine kinase receptor which is required for the formation of the neuromuscular junction. Antibodies against MuSK inhibit the signaling of MuSK normally induced by its nerve-derived ligand, agrin. The result is a decrease in patency of the neuromuscular junction, and the consequent symptoms of MG.

People treated with penicillamine can develop MG symptoms. Their antibody titer is usually similar to that of MG, but both the symptoms and the titer disappear when drug administration is discontinued.

MG is more common in families with other autoimmune diseases. A familial predisposition is found in 5% of the cases. This is associated with certain genetic variations such as an increased frequency of HLA-B8 and DR3. People with MG also have an increased risk of developing another autoimmune disease.

Diagnosis

Myasthenia can be a difficult diagnosis, as the symptoms can be subtle and hard to distinguish from both normal variants and other neurological disorders.^[3] A patient may have visited the ENT doctor, the ophthalmologist and even the psychiatrist and waited for years for the right diagnosis.

A thorough physical examination can reveal easy fatiguability, with the weakness improving after rest and worsening again on repeat of the exertion testing. Though this is not often performed, applying ice to the weak muscle groups characteristically improves the weakness. Additional tests are often performed, as mentioned below. Furthermore, a good response to medication can also be considered a sign of autoimmune pathology.

Physical examination

Muscle fatigability can be tested for many muscles. A thorough investigation includes

• looking upward and sidewards for 30 seconds: ptosis and diplopia.
• looking at the feet while lying on the back for 60 seconds
• keeping the arms stretched forward for 60 seconds
• 10 deep knee bends
• walking 30 steps on both the toes and the heels
• 5 situps, lying down and sitting up completely

Blood tests

If the diagnosis is suspected, serology can be performed in a blood test to identify antibodies against the acetylcholine receptor. The test has a reasonable sensitivity of 80–96%, but in MG limited to the eye muscles (ocular myasthenia) the test may be negative in up to 50% of the cases. About half of the patients without antibodies against the acetylcholine receptor have antibodies against the MuSK protein. Often, parallel testing is performed for Lambert-Eaton syndrome, in which other antibodies (against a voltage-gated calcium channel) can be found. Also the thyroid function should be tested.

Single-fiber electromyography and repetitive nerve stimulation

Muscle fibers of patients with MG are easily fatigued, and thus do not respond as well as muscles in healthy individuals to repeated stimulation. By repeatedly stimulating a muscle with electrical impulses, the fatiguability of the muscle can be measured. This is called the repetitive nerve stimulation test. In single fiber electromyography, which is considered to be the most sensitive (although not the most specific) test for MG, a thin needle electrode is inserted into a muscle to record the electric potentials of individual muscle fibers. By finding two muscle fibers belonging to the same motor unit and measuring the temporal variability in their firing patterns (i.e. their 'jitter'), the diagnosis can be made.

Edrophonium test

The "edrophonium test" is infrequently performed to identify MG; its application is limited to the situation when other investigations do not yield a conclusive diagnosis. This test requires the intravenous administration of edrophonium chloride (Tensilon®, Reversol®), a drug that blocks the breakdown of acetylcholine by cholinesterase and temporarily increases the levels of acetylcholine at the neuromuscular junction. In people with myasthenia gravis involving the eye muscles, edrophonium chloride will briefly relieve weakness.

Imaging

A chest X-ray is frequently performed; it may point towards alternative diagnoses (e.g. Lambert-Eaton due to a lung tumor) and comorbidity. It may also identify widening of the mediastinum suggestive of thymoma, but computed tomography (CT) or magnetic resonance imaging (MRI) are more sensitive ways to identify thymomas, and are generally done for this reason.

Pulmonary function test

Spirometry (lung function testing) may be performed to assess respiratory function if there are concerns about a patient's ability to breathe adequately. The FEV1 (forced expired volume in one second) or the PEFR (peak expiratory flow rate) may be monitored at intervals in order not to miss a gradual worsening of muscular weakness. Severe myasthenia may cause respiratory failure due to exhaustion of the respiratory muscles.

Pathological findings

Immunofluoresence shows IgG antibodies on the neuromuscular junction. (Note that it is not the antibody which causes myasthenia gravis that fluoresces, but rather a secondary antibody directed against it.) Muscle electron microscopy shows receptor infolding and loss of the tips of the folds, together with widening of the synaptic clefts. Both these techniques are currently used for research rather than diagnostically.

Associations

As Myasthenia gravis is an autoimmune condition, there are several other diseases that it is associated with that need to be investigated for: -SLE -Hashimoto's thyroiditis

Treatment

Myasthenia gravis can usually be controlled with medication. Medication is used for two different endpoints:

• Direct improvement of the weakness
• Reduction of the autoimmune process

Muscle function is improved by cholinesterase inhibitors, such as neostigmine and pyridostigmine. These slow the natural enzyme cholinesterase that degrades acetylcholine in the motor end plate; the neurotransmitter is therefore around longer to stimulate its receptor. Usually doctors will start with a low dose, eg 3x20mg pyridostigmine, and increase until the desired result is achieved. If taken 30 minutes before a meal, symptoms will be mild during eating. Side effects, like perspiration and diarrhea can be countered by adding atropine. Pyridostigmine is a short-lived drug with a half-life of about 4 hours. Immunosuppressive drugs such as prednisone, cyclosporine, mycophenolate mofetil and azathioprine may be used. It is common for patients to be treated with a combination of these drugs with a cholinesterase inhibitor. Treatments with some immunosuppressives take weeks to months before effects are noticed. If the myasthenia is serious (myasthenic crisis), plasmapheresis is used to remove the putative antibody from the circulation. Similarly, intravenous immunoglobulins (IVIg) is used to bind the circulating antibodies. Both of these treatments have relatively short-lived benefits, typically measured in weeks.

Thymectomy, the surgical removal of the thymus gland (which is abnormal in myasthenia gravis patients), improves symptoms in more than 50 percent of patients. Some patients are cured by thymectomy, suggesting that the thymus plays a significant role in the pathogenesis of myasthenia. The positive effects from a thymectomy may be seen within weeks to as much as 3–5 years after surgery.

There are a number of surgical approaches to the removal of the thymus gland: transsternal (through the breast bone), transcervical (through a small neck incision), transthoracic (through one or both sides of the chest). The transsternal approach is most common and uses the same length-wise incision through the sternum (breast bone)used for most open-heart surgery. It is espoused by surgeons such as Alfred Jaretzki and is the most commonly performed procedure due to its relative simplicity. The transcervical approach is a less invasive procedure that allows for removal of the entire thymus gland through a small neck incision. It has been popularized by Joel Cooper. Because of its increased technical demands, it is performed by only a relative few surgeons in North America: Joel Cooper and Larry Kaiser (University of Pennsylvania; Philadelphia, Pennsylvania), Bryan Meyers (Washington University; St. Louis, Missouri), Stephen Cassivi (Mayo Clinic; Rochester, Minnesota), Sudhir Sundaresan (University of Ottawa; Ottawa, Canada), Shaf Keshavjee (University of Toronto; Toronto, Canada). Interestingly, there has been no difference in success in symptom improvement between the transsternal approach and the minimally invasive transcervical approach.^[6]

Thymoma is relatively rare in younger (<40) patients, but paradoxically especially younger patients with generalized MG without thymoma benefit from thymectomy. Of course resection is also indicated for those with a thymoma, but it is less likely to improve the MG symptoms.

Prognosis

With treatment, patients have a normal life expectancy, except for those with a malignant thymoma (whose lesser life expectancy is on account of the thymoma itself and is otherwise unrelated to the myasthenia). Quality of life can vary depending on the severity and the cause. The drugs used to control MG either diminish in effectiveness over time (cholinesterase inhibitors) or cause severe side effects of their own (immunosupressants). A small percentage (around 10%) of MG patients are found to have tumors in their Thymus, in which case Thymectomy is a very effective treatment with long term remission. However, most patients need treatment for the remainder of their lives, and their abilities vary greatly. It should be noted that MG is not a progressive disease. The symptoms may come and go, but the symptoms usually don't get worse as the patient ages. For some, the symptoms decrease after 3–5 years.

Epidemiology

Myasthenia gravis occurs in all ethnic groups and both genders. It most commonly affects women under 40 and people from 50 to 70 years old of both sexes, but it can occur at any age. Younger patients rarely have thymoma. The prevalence in the United States is estimated at 20 cases per 100,000 in the USA.^[1] Risk factors are female gender, age 20–40, familial myasthenia gravis, D-penicillamine ingestion (drug induced myasthenia) and other autoimmune disease.

Three types of myasthenia symptoms in children can be distinguished:

1. Neonatal: In 12% of the pregnancies with a mother with MG, she passes the antibodies to the infant through the placenta causing neonatal myasthenia gravis. The symptoms will start in the first two days and disappear within a few weeks after birth. With the mother it is not uncommon for the symptoms to even improve during pregnancy, but they might worsen after labor.
2. Congenital: Children of a healthy mother can, very rarely, develop myasthenic symptoms beginning at birth. This is called Congenital Myasthenic Syndrome or CMS. Other than Myasthenia gravis, CMS is not caused by an autoimmune process, but due to synaptic malformation, which in turn is caused by genetic mutations. Thus, CMS is a hereditary disease. More than 11 different mutations have been identified and the inheritance pattern is typically autosomal recessive.

The congenital myasthenias cause muscle weakness and fatigability similar to those of MG. The symptoms of CMS usually begin within the first two years of life, although in a few forms patients can develop their first symptoms as late as the seventh decade of life. A diagnosis of CMS is suggested by the following:

• Onset of symptoms in infancy or childhood.
• Weakness which increases as muscles tire.
• A decremental EMG response, on low frequency, of the compound muscle action potential (CMAP).
• No anti-AChR or MuSK antibodies.
• No response to immunosuppressant therapy.
• Family history of symptoms which resemble CMS.

The symptoms of CMS vary from mild to severe, depending on the form. It's also common for patients with the same form, even members of the same family, to be affected to differing degrees. In most forms of CMS weakness does not progress, and in some forms symptoms may diminish as the patient gets older. Only rarely do symptoms of CMS become worse with time.

1. Juvenile myasthenia gravis: Myasthenia occurring at a young age.

Notes

References

• Baets, MH de, Oosterhuis HJGH. Myasthenia gravis. Boca Raton: DRD Press, 1993
• Rowland LP, ed: Merritt's textbook of Neurology. 10th Ed. Philadelphia, Lippincott, Williams & Wilkins, 1995
• Cavel-Greant, D, Nicolle, MW ed; You, Me and Myasthenia Gravis Third ed. Ku:Reh Press, 2006

External links

• One of the world's largest treatment centers - The Myasthenia Gravis Association of Western PA
• The Myasthenia Gravis Foundation of America
• The Myasthenia Gravis Association (MGA) in the United Kingdom & the Republic of Ireland
• Minimally Invasive Transcervical Thymectomy - educational information with description and images of procedure by Mayo Clinic
• Website for You, Me and Myasthenia Gravis, third ed. - includes two freely available chapters
• Myasthenia Gravis Center at the University of Maryland Medical Center
• Myasthenia Gravis(Turkish)

•  • [ e] Nervous system pathology, primarily PNS (, )
Nerve, nerve root and plexus disorders	cranial nerve: V (Trigeminal neuralgia) - VII (Facial nerve paralysis, Bell's palsy, Melkersson-Rosenthal syndrome, Central seven) - XI (Accessory nerve disorder) nerve root and plexus: Brachial plexus lesion - Thoracic outlet syndrome - Phantom limb mononeuropathy: Carpal tunnel syndrome - Ulnar nerve entrapment - Radial neuropathy - Causalgia - Meralgia paraesthetica - Tarsal tunnel syndrome - Morton's neuroma - Mononeuritis multiplex
Polyneuropathies and other disorders of the PNS	Hereditary and idiopathic (Charcot-Marie-Tooth disease, Dejerine Sottas syndrome, Refsum's disease, Morvan's syndrome) - Guillain-Barr syndrome - Alcoholic polyneuropathy - Neuropathy
Diseases of myoneural junction and muscle	Myasthenia gravis - Primary disorders of muscles (Muscular dystrophy, Myotonic dystrophy, Myotonia congenita, Thomsen disease, Neuromyotonia, Paramyotonia congenita, Centronuclear myopathy, Nemaline myopathy, Mitochondrial myopathy) - Myopathy - Periodic paralysis (Hypokalemic, Hyperkalemic) - Lambert-Eaton myasthenic syndrome
Autonomic	Familial dysautonomia - Horner's syndrome - Multiple system atrophy (Shy-Drager syndrome, Olivopontocerebellar atrophy)