Information about Interactome
Interactome is the whole set of molecular interactions in cells. It is usually displayed as a directed graph. When spoken in terms of proteomics, it refers to protein-protein interaction network(PPI), or protein network (PN).
Experimental methods have been devised to determine PPI, such as 1) affinity purification and 2) yeast two hybrid (Y2H). The former is suited to identify a protein complex, while the latter is suited to explore the binary interactions in mass quantities. The former is considered as a low-throughput method (LTP), while the latter is considered as high-throughput method (HTP).
There have been several efforts to map the eukaryotic interactome through HTP methods. Yeast, fly, worm, and human HTP maps have been created so far (2006).
Methods of mapping the interactome
The study of the interactome is called interactomics. The basic unit of protein network is protein-protein interaction (PPI). Because the interactome considers the whole organism, there is a need to collect a massive amount of information.Experimental methods have been devised to determine PPI, such as 1) affinity purification and 2) yeast two hybrid (Y2H). The former is suited to identify a protein complex, while the latter is suited to explore the binary interactions in mass quantities. The former is considered as a low-throughput method (LTP), while the latter is considered as high-throughput method (HTP).
There have been several efforts to map the eukaryotic interactome through HTP methods. Yeast, fly, worm, and human HTP maps have been created so far (2006).
Interactome databases
- Database of interacting protein (DIP) (Xenarios et al. 2000)
- GRID database
- MIPS database
- PSIMAP database: The first protein structural interactome DB
- InterPare database: A structural protein interfaceome DB
- Biomolecular Interaction Network Database (BIND) (Bader et al, 2003)
- Online Predicted Human Interaction Database (OPHID) (Brown and Jurisica, 2005)
- Human Protein Reference Database (HPRD) (Peri, S. et al, 2003)
- Himap(Human Interactome Map)
- HPID
- MINT
- PINdb
- IntAct - The Molecular Interaction Database
- APID - Agile Protein Interaction DataAnalyzer APID is interactive bioinformatic web-tool that integrates and unifies main known experimentally validated protein-protein interactions.
See also
External links
References
- Bader GD, et al. (2003) BIND: The Biomolecular interaction Network Database. Nucleic Acids Res., 31, 248-50.
- Brown KR, Jurisica I. (2005) Online Predicted Human Interaction Database. Bioinformatics, 21(9): 2076-82.
- Xenarios I, Rice DW, Salwinski L, Baron MK, Marcotte EM, Eisenberg D. (2000) DIP: the database of interacting proteins. Nucleic Acids Res. 28: 289-91.
- Peri S., Navarro JD, Amanchy R, Kristiansen TZ, Jonnalagadda CK, et al. (2003) Development of human protein reference database as an initial platform for approaching systems biology in humans. Genome Res 13:2363-71.
- Park J, Lappe M, Teichmann SA. (2001) Mapping protein family interactions: intramolecular and intermolecular protein family interaction repertoires in the PDB and yeast. J Mol Biol. 2001 Mar 30;307(3):929-38.
Proteomics is the large-scale study of proteins, particularly their structures and functions.[1][2] Proteins are vital parts of living organisms, as they are the main components of the physiological pathways of cells.
..... Click the link for more information.
..... Click the link for more information.
Protein-protein interactions refer to the association of protein molecules and the study of these associations from the perspective of biochemistry, signal transduction and networks.
The interactions between proteins are important for many biological functions.
..... Click the link for more information.
The interactions between proteins are important for many biological functions.
..... Click the link for more information.
Interactomics is a discipline of bioinformatics that deals with studying the interactome, which is the interaction among proteins, and other molecules within a cell.
..... Click the link for more information.
..... Click the link for more information.
Bioinformatics and computational biology involve the use of techniques including applied mathematics, informatics, statistics, computer science, artificial intelligence, chemistry, and biochemistry to solve biological problems usually on the molecular level.
..... Click the link for more information.
..... Click the link for more information.
This is a navigational and informational list.
The terms 'Ome' and 'Omics' are derivations of the suffix -ome, which has been appended to a variety of previously existing biological terms to create names for fields of endeavor that are either speculative or have some
..... Click the link for more information.
The terms 'Ome' and 'Omics' are derivations of the suffix -ome, which has been appended to a variety of previously existing biological terms to create names for fields of endeavor that are either speculative or have some
..... Click the link for more information.
This article is copied from an article on Wikipedia.org - the free encyclopedia created and edited by online user community. The text was not checked or edited by anyone on our staff. Although the vast majority of the wikipedia encyclopedia articles provide accurate and timely information please do not assume the accuracy of any particular article. This article is distributed under the terms of GNU Free Documentation License.
Herod_Archelaus
